Eating food off the floor is not safe as bacteria may transfer in less than a second, says a new study that disproves the widely accepted notion that it is alright to scoop up food within a "safe" five-second window.
Professor Donald Schaffner from Rutgers University in the US found that moisture, type of surface and contact time all contribute to cross-contamination.
In some instances, the transfer begins in less than one second, he said.
"The popular notion of the 'five-second rule' is that food dropped on the floor, but picked up quickly, is safe to eat because bacteria need time to transfer," Schaffner said, adding that while the pop culture "rule" has been featured in at least two TV programmes, research in peer-reviewed journals is limited.
The researchers tested four surfaces - stainless steel, ceramic tile, wood and carpet - and four different foods (watermelon, bread, bread and butter, and gummy candy).
They also looked at four different contact times - less than one second, five, 30 and 300 seconds.
They used two media - tryptic soy broth or peptone buffer - to grow Enterobacter aerogenes, a nonpathogenic "cousin" of Salmonella naturally occurring in the human digestive system.
Transfer scenarios were evaluated for each surface type, food type, contact time and bacterial prep; surfaces were inoculated with bacteria and allowed to completely dry before food samples were dropped and left to remain for specified periods.
All totalled 128 scenarios were replicated 20 times each, yielding 2,560 measurements. Post-transfer surface and food samples were analysed for contamination.
Not surprisingly, watermelon had the most contamination, gummy candy the least, researchers found.
"Transfer of bacteria from surfaces to food appears to be affected most by moisture," said Schaffner, who conducted the study with Robyn Miranda, a graduate student in his laboratory at Rutgers.
"Bacteria don't have legs, they move with the moisture, and the wetter the food, the higher the risk of transfer.
Also, longer food contact times usually result in the transfer of more bacteria from each surface to food," said Schaffner.
Carpet has unexpectedly very low transfer rates compared with those of tile and stainless steel, whereas transfer from wood is more variable, researchers said.
"The topography of the surface and food seem to play an important role in bacterial transfer," Schaffner said.
While the research demonstrates that the five-second rule is "real" in the sense that longer contact time results in more bacterial transfer, it also shows other factors, including the nature of the food and the surface it falls on, are of equal or greater importance.
"The five-second rule is a significant oversimplification of what actually happens when bacteria transfer from a surface to food," Schaffner said.
"Bacteria can contaminate instantaneously," he added.
